Activation of Imidazoline I-2B Receptors by Allantoin to Increase Glucose Uptake into C2C12 Cells

M. F. Chen; T. T. Yang; L. R. Yeh; H. H. Chung; Y. J. Wen; W. J. Lee; J. T. Cheng

doi:10.1055/s-0032-1301898

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2012; 44(04): 268-272
DOI: 10.1055/s-0032-1301898

Original Basic

Activation of Imidazoline I-2B Receptors by Allantoin to Increase Glucose Uptake into C2C12 Cells

M. F. Chen

¹Department of Nursing and Biotechnology, Chung Hwa University of Medical Technology, Jen-Te, Tainan City, Taiwan

,

T. T. Yang

²Department of Pathology, School of Chinese Medicine for Post-Baccalaureate, and Department of Radiology in E-Da Hospital, I-Shou University, Kaohsiung City, Taiwan

,

L. R. Yeh

²Department of Pathology, School of Chinese Medicine for Post-Baccalaureate, and Department of Radiology in E-Da Hospital, I-Shou University, Kaohsiung City, Taiwan

,

H. H. Chung

³Institute of Basic Medical Sciences and Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan

,

Y. J. Wen

³Institute of Basic Medical Sciences and Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan

,

W. J. Lee

⁴Department of Emergency Medicine and Department of Medical Research, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan

,

J. T. Cheng

³Institute of Basic Medical Sciences and Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan

⁴Department of Emergency Medicine and Department of Medical Research, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan

⁵Institute of Medical Science, College of Health Science, Chang Jung Christian University, Guei-Ren, Tainan City, Taiwan

› Institutsangaben

Abstract

Allantoin, an active principle of the yam, belongs to the group of guanidinium derivatives and has been reported to lower plasma glucose in diabetic animals. Recent evidence indicates that activation of the imidazoline I_2B receptor (I_2BR) by guanidinium derivatives also increases glucose uptake; however, the effect of allantoin on I_2BR is still unknown. Glucose uptake into cultured C₂C₁₂ cells was determined using 2-[¹⁴C]-deoxy-d-glucose as a tracer. The changes in 5′-AMP-activated protein kinase (AMPK) expression were also identified by Western blotting analysis. The allantoin-induced glucose uptake action was dose-dependently blocked by BU224, a specific I₂R antagonist, in C₂C₁₂ cells. Moreover, AMPK phosphorylation by allantoin was found to be dose-dependently increased in C₂C₁₂ cells using AICAR treatment as a reference. In addition, both actions of allantoin, the increases in glucose uptake and AMPK phosphorylation, were dose-dependently attenuated by amiloride in C₂C₁₂ cells. Moreover, compound C at concentrations sufficient to inhibit AMPK blocked the allantoin-induced glucose uptake and AMPK phosphorylation. Thus, we suggest that allantoin can activate I_2BR to increase glucose uptake into cells, and propose I_2BR as a new target for diabetic therapy.

Key words

allantoin - 5′-AMP-activated protein kinase - BU224 - glucose uptake - imidazoline receptor

Volltext

Referenzen

References
1 Sagara K, Ojima M, Suto K, Yoshida T. Quantitative determination of allantoin in Dioscorea rhizome and an Oriental pharmaceutical preparation, hachimi-gan, by high-performance liquid chromatography. Planta Med 1989; 55: 93
2 Shestopalov AV, Shkurat TP, Mikashinovich ZI, Kryzhanovskaia IO, Bogacheva MA, Lomteva SV, Prokofev VP, Guskov EP. Biological functions of allantoin. Izv Akad Nauk Ser Biol 2006; 541-545
3 Sato K, Iemitsu M, Aizawa K, Ajisaka R. DHEA improves impaired activation of Akt and PKC zeta/lambda-GLUT4 pathway in skeletal muscle and improves hyperglycaemia in streptozotocin-induced diabetes rats. Acta Physiol 2009; 197: 217-225
4 Gao X, Li B, Jiang H, Liu F, Xu D, Liu Z. Dioscorea opposita reverses dexamethasone induced insulin resistance. Fitoterapia 2007; 78: 12-15
5 Hsu JH, Wu YC, Liu IM, Cheng JT. Dioscorea as the principal herb of Die-Huang-Wan, a widely used herbal mixture in China, for improvement of insulin resistance in fructose-rich chow-fed rats. J Ethnopharmacol 2007; 112: 577-584
6 Shujun W, Jinglin Y, Wenyuan G, Hongyan L, Peigen X. New starches from traditional Chinese medicine (TCM) – Chinese yam (Dioscorea opposita Thunb.) cultivars. Carbohydr Res 2006; 341: 289-293
7 Niu CS, Chen W, Wu HT, Cheng KC, Wen YJ, Lin KC, Cheng JT. Decrease of plasma glucose by allantoin, an active principle of yam (Dioscorea spp.), in streptozotocin-induced diabetic rats. J Agric Food Chem 2010; 58: 12031-12035
8 Kim YD, Park KG, Lee YS, Park YY, Kim DK, Nedumaran B, Jang WG, Cho WJ, Ha J, Lee IK, Lee CH, Choi HS. Metformin inhibits hepatic gluconeogenesis through AMP-activated protein kinase-dependent regulation of the orphan nuclear receptor SHP. Diabetes 2008; 57: 306-314
9 Sakamoto K, McCarthy A, Smith D, Green KA, Grahame Hardie D, Ashworth A, Alessi DR. Deficiency of LKB1 in skeletal muscle prevents AMPK activation and glucose uptake during contraction. EMBO J 2005; 24: 1810-1820
10 Lui TN, Tsao CW, Huang SY, Chang CH, Cheng JT. Activation of imidazoline I2B receptors is linked with AMP kinase pathway to increase glucose uptake in cultured C2C12 cells. Neurosci Lett 2010; 474: 144-147
11 Dardonville C, Rozas I. Imidazoline binding sites and their ligands: an overview of the different chemical structures. Med Res Rev 2004; 24: 639-661
12 Michel MC, Insel PA. Are there multiple imidazoline binding sites?. Trends Pharmacol Sci 1989; 10: 342-344
13 Bousquet P, Feldman J, Schwartz J. Central cardiovascular effects of alpha adrenergic drugs: differences between catecholamines and imidazolines. J Pharmacol Exp Ther 1984; 230: 232-236
14 Morgan NG, Chan SL, Mourtada M, Monks LK, Ramsden CA. Imidazolines and pancreatic hormone secretion. Ann N Y Acad Sci 1999; 881: 217-228
15 Meana JJ, Barturen F, Martin I, Garcia-Sevilla JA. Evidence of increased non-adrenoceptor [3H]idazoxan binding sites in the frontal cortex of depressed suicide victims. Biol Psychiatry 1993; 34: 498-501
16 Boronat MA, Olmos G, Garcia-Sevilla JA. Attenuation of tolerance to opioid-induced antinociception by idazoxan and other I2-ligands. Ann N Y Acad Sci 1999; 881: 359-363
17 Yang PS, Lee JJ, Tsao CW, Wu HT, Cheng JT. Stimulatory effect of stevioside on peripheral mu opioid receptors in animals. Neurosci Lett 2009; 454: 72-75
18 Chen CT, Chen W, Chung HH, Cheng KC, Yeh CH, Cheng JT. Activation of imidazoline I-2B receptor by metformin to increase glucose uptake in skeletal muscle. Horm Metab Res 2011; 43: 708-713
19 Chang CH, Tsao CW, Huang SY, Cheng JT. Activation of imidazoline I(2B) receptors by guanidine to increase glucose uptake in skeletal muscle of rats. Neurosci Lett 2009; 467: 147-149
20 Yang TT, Liu IM, Wu HT, Cheng JT. Mediation of protein kinase C zeta in mu-opioid receptor activation for increase of glucose uptake into cultured myoblast C2C12 cells. Neurosci Lett 2009; 465: 177-180
21 Hsu JH, Liou SS, Yu BC, Cheng JT, Wu YC. Activation of alpha1A-adrenoceptor by andrographolide to increase glucose uptake in cultured myoblast C2C12 cells. Planta Med 2004; 70: 1230-1233
22 Yuan Y, Shi XE, Liu YG, Yang GS. FoxO1 regulates muscle fiber-type specification and inhibits calcineurin signaling during C2C12 myoblast differentiation. Mol Cell Biochem 2011; 348: 77-87
23 Bajaj P, Reddy Jr B, Millet L, Wei C, Zorlutuna P, Bao G, Bashir R. Patterning the differentiation of C2C12 skeletal myoblasts. Integr Biol (Camb) 2011; 3: 897-909
24 Sarabia V, Lam L, Burdett E, Leiter LA, Klip A. Glucose transport in human skeletal muscle cells in culture. Stimulation by insulin and metformin. J Clin Invest 1992; 90: 1386-1395
25 Diamant S, Eldar-Geva T, Atlas D. Imidazoline binding sites in human placenta: evidence for heterogeneity and a search for physiological function. Br J Pharmacol 1992; 106: 101-108
26 Lin KC, Yeh LR, Chen LJ, Wen YJ, Cheng KC, Cheng JT. Plasma Glucose-lowering Action of Allantoin is Induced by Activation of Imidazoline I-2 Receptors in Streptozotocin-induced Diabetic Rats. Horm Metab Res 2012; 44: 41-46
27 MacInnes N, Handley SL. Potential serotonergic and noradrenergic involvement in the discriminative stimulus effects of the selective imidazoline I2-site ligand 2-BFI. Pharmacol Biochem Behav 2003; 75: 427-433
28 Chang CH, Wu HT, Cheng KC, Lin HJ, Cheng JT. Increase of beta-endorphin secretion by agmatine is induced by activation of imidazoline I(2A) receptors in adrenal gland of rats. Neurosci Lett 2010; 468: 297-299
29 Mu J, Brozinick Jr JT, Valladares O, Bucan M, Birnbaum MJ. A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle. Mol Cell 2001; 7: 1085-1094
30 Zong H, Ren JM, Young LH, Pypaert M, Mu J, Birnbaum MJ, Shulman GI. AMP kinase is required for mitochondrial biogenesis in skeletal muscle in response to chronic energy deprivation. Proc Natl Acad Sci USA 2002; 99: 15983-15987
31 Zheng D, MacLean PS, Pohnert SC, Knight JB, Olson AL, Winder WW, Dohm GL. Regulation of muscle GLUT-4 transcription by AMP-activated protein kinase. J Appl Physiol 2001; 91: 1073-1083
32 Watson RT, Kanzaki M, Pessin JE. Regulated membrane trafficking of the insulin-responsive glucose transporter 4 in adipocytes. Endocr Rev 2004; 25: 177-204
33 Liu XJ, He AB, Chang YS, Fang FD. Atypical protein kinase C in glucose metabolism. Cell Signal 2006; 18: 2071-2076
34 Limon I, Coupry I, Lanier SM, Parini A. Purification and characterization of mitochondrial imidazoline-guanidinium receptive site from rabbit kidney. J Biol Chem 1992; 267: 21645-21649
35 Raddatz R, Parini A, Lanier SM. Localization of the imidazoline binding domain on monoamine oxidase B. Mol Pharmacol 1997; 52: 549-553
36 Carpene C, Collon P, Remaury A, Cordi A, Hudson A, Nutt D, Lafontan M. Inhibition of amine oxidase activity by derivatives that recognize imidazoline I2 sites. J Pharmacol Exp Ther 1995; 272: 681-688